Gontzal García del Caño

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Questions related from Gontzal García del Caño

Can anyone help me to find a mammalian expression vector for high transfection efficiency and mild expression at once?

We're currently using pCDNA vector to induce protein overexpression in the Ntera2 cell line and having problems with cell survival after transfection with plasmids containing the insert for our...

01 December 2016 9,564 1 View

Can anyone recommend how to prepare matrigel?

I'm working on a model of neuronal differentiation and use matrigel use as a substrate. Does anyone have an exact idea about how should it be prepared?. Should it be allowed to dry after putting...

04 October 2012 8,647 9 View

Reliable and proven method to analyze cell viability and proliferation in culture by microscopy?

I'm performing transfections with shRNA in cultured cells for specific knock down of a protein of interest. I suspect that knocking down promotes proliferation or maybe by scramble control...

11 September 2012 2,992 30 View