Does anyone have experience with optiprep gradient ultracentrifugation in 5-ml tubes (SW55 Ti rotor)?

I've been trying to isolate extracellular vesicles on an optiprep gradient in 5-ml tubes. I've tried 2 and 12 h centrifugation and in both cases, the fractions seemed to be mixed after...

07 July 2016 3,344 5 View