Golnaz Morad

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Questions related from Golnaz Morad

Which method gives a higher yield for astrocyte isolation from adult mouse brain? FACS or bead magnetic separation?

I'm planning to isolate astrocytes from adult mouse brain (whole brain) to evaluate protein expression, etc. (no culture or expansion). I need a method that isolates a pure population with high...

12 September 2017 8,144 0 View

Which method is better for exosome isolation from condition media? Ultracentrifugation or sucrose gradient ultracentrifugation?

I'm collecting exosomes from condition media for proteomics purposes. Is adding the extra step of sucrose gradient UC essential for getting an acceptably pure sample? If so, what protocol do you...

08 September 2014 1,033 17 View