Golla Ramanjaneyulu

20 Questions 9 Answers 0 Followers

Questions related from Golla Ramanjaneyulu

How to clone ?

Hello, If I want to clone a gene of interest in a plasmid containing ORF, how should I do that, and which criteria I should follow? Thanks, Ramanjaneyulu

15 May 2020 9,646 2 View

How to design a primer?

Hello, If the gene our interest to amplify does not follow any criteria in designing primers how to proceed with that sequence? If the gene sequence is1000 - 1500 bp. Thank you, Ramanjaneyulu

15 May 2020 1,677 15 View

How to calculate saccharification %?

Hello Every one, I have done saccharification with 50 U/ml crude enzyme extract in 50 ml buffer with 5% solid loading. Now I have a doubt at calculation part. If I get released reducing sugar of...

21 March 2016 9,775 10 View

How can one become able to select particular concentration of enzyme in U/ml?

Hello Every one, I am working on saccharification of biomass with crude enzyme. In this case I need to select certain concentration of enzyme (eg: 500 U/ ml), If suppose my enzyme...

19 November 2015 6,028 2 View

What is a bootstrap value?

What is bootstrap value? what does it denotes? Is it necessary for the construction of phylogenetic tree and what is the best no. of bootstrap replicates value for construction of fungal...

13 July 2015 2,494 16 View

Which is the best on-line database for the construction of a phylogenetic tree for fungi?

Hello Every one, I have isolated and sequenced ITS regions for identification of fungi by Sangers method. To construct a phylogenetic tree which is the best tool available in on-line...

09 March 2015 9,617 13 View

How can I optimize culture conditions of enzyme production by using Response Surface Methodology?

Hello Every one, I have done experiments on enzyme production and optimization of production parameters like temp, pH, ...etc. Now I have to optimize the significant parameters by using Response...

07 January 2015 6,351 4 View

What is the best method to isolate/extract fungal genomic DNA?

What is the best method to isolate/extract fungal genomic DNA? I have tried several methods without the addition of chitinase, but I wasn't successful in this aspect. Please suggest a better...

11 December 2014 7,632 9 View

How do I isolate yeast from soil?

Which is the best dilution to isolate yeast from soil while doing serial dilution method? Please let me know the details. Thank you.

26 November 2014 2,534 3 View

What is the role of acids in lignocellulosic biomass pretreatment?

Hi all,May I know what is the role of acids (HCl, H2SO4, Acetic acid, phosphoric acid, Oxalic acid and nitric acid) in removing lignin/ cellulose/ hemicellulose in lignocellulosic biomass?If we...

13 October 2014 6,014 10 View

Is there any method for detection of Glu, Xylose and Xylitol by HPLC?

Hello Everyone, Please let me know, if there is any single or different methods available for detection of glucose, xylose and xylitol by HPLC equipped with C18 column and UV detector. If not...

20 August 2014 8,919 14 View

How does one quantify Cellulose, Hemicellulose and Lignin?

How does one quantify total cellulose, hemicellulose, and lignin in biomass before pre-treatment of lignocellulosic biomass?

18 August 2014 3,017 22 View

How one can analyse the biomass to quantify the reducing sugars?

Lignocellulose is a renewable biomass which is widely available in nature. It contains cellulose (glucose), hemicellulose (xylose, arabinose, mannose etc.) and lignin. How one can analyse a...

16 August 2014 7,040 5 View

How to find the synthesized derivatives of natural compounds which are synthesized earlier or not?

We are planning to synthesize the new derivatives of natural compound (Ex-curcumin) has not yet synthesized elsewhere. Hence, please let me know the tools to find out all derivatives available for...

18 July 2014 4,989 3 View

How can I predict the insilico ADMET (toxicity) of a new drug?

We are designing new drugs using online molinspiration program for that we want check the ADMET in silico. What are the standards for ADMET of a designed drug. Are there any software's...

11 July 2014 8,249 27 View

Why didn't I get linear chromatogram in GC?

I am running standards for Gas Chromatography with various concentrations like 0.5, 1.0, 1.5 mg/ml of ethanol dissolved in distilled water (HPLC). I am getting single peak but area is not constant...

30 May 2014 5,251 11 View

What are the best universal primers to amplify 18s rRNA of fungal origin?

I have isolated some fungi from soil and now I am trying to identify the species. For the identification what are the best primers (Universal/Other) to amplify fungal DNA and where I can get them?...

23 May 2014 6,232 8 View

Is it possible to detect xylitol with GC?

Is it possible to detect xylitol with Gas Chromatography (GC)? If it's possible what are the conditions for detection of xylitol with GC? We have a GC with capillary column and FID detector. What...

22 May 2014 7,144 5 View

How can I detect ethanol using gas chromatography?

Please let me know how I can detect ethanol in the fermentation media. Is there any specific methodology (injector, column temp, detector temp) for the detection of ethanol in the media? And do we...

27 January 2014 5,264 6 View

Does anyone have experience with biomass (Lignocellulose) pre-treatment?

I am working on bioethanol production. How can I quantify the reducing sugars percentage (glucose, xylose, arabinose,galactose, etc.) by using HPLC, after pretreatment, and what are the column...

08 July 2013 9,269 3 View