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Questions related from Golla Ramanjaneyulu
Hello, If I want to clone a gene of interest in a plasmid containing ORF, how should I do that, and which criteria I should follow? Thanks, Ramanjaneyulu
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Hello, If the gene our interest to amplify does not follow any criteria in designing primers how to proceed with that sequence? If the gene sequence is1000 - 1500 bp. Thank you, Ramanjaneyulu
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Hello Every one, I have isolated and sequenced ITS regions for identification of fungi by Sangers method. To construct a phylogenetic tree which is the best tool available in on-line...
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What is the best method to isolate/extract fungal genomic DNA? I have tried several methods without the addition of chitinase, but I wasn't successful in this aspect. Please suggest a better...
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Hi all,May I know what is the role of acids (HCl, H2SO4, Acetic acid, phosphoric acid, Oxalic acid and nitric acid) in removing lignin/ cellulose/ hemicellulose in lignocellulosic biomass?If we...
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Hello Everyone, Please let me know, if there is any single or different methods available for detection of glucose, xylose and xylitol by HPLC equipped with C18 column and UV detector. If not...
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Is it possible to detect xylitol with Gas Chromatography (GC)? If it's possible what are the conditions for detection of xylitol with GC? We have a GC with capillary column and FID detector. What...
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I am working on bioethanol production. How can I quantify the reducing sugars percentage (glucose, xylose, arabinose,galactose, etc.) by using HPLC, after pretreatment, and what are the column...
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