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Questions related from Golla Ramanjaneyulu
Hello, If I want to clone a gene of interest in a plasmid containing ORF, how should I do that, and which criteria I should follow? Thanks, Ramanjaneyulu
15 May 2020 9,548 2 View
Hello, If the gene our interest to amplify does not follow any criteria in designing primers how to proceed with that sequence? If the gene sequence is1000 - 1500 bp. Thank you, Ramanjaneyulu
15 May 2020 1,558 15 View
Hello Every one, I have done saccharification with 50 U/ml crude enzyme extract in 50 ml buffer with 5% solid loading. Now I have a doubt at calculation part. If I get released reducing sugar of...
21 March 2016 9,720 10 View
Hello Every one, I am working on saccharification of biomass with crude enzyme. In this case I need to select certain concentration of enzyme (eg: 500 U/ ml), If suppose my enzyme...
19 November 2015 5,987 2 View
What is bootstrap value? what does it denotes? Is it necessary for the construction of phylogenetic tree and what is the best no. of bootstrap replicates value for construction of fungal...
13 July 2015 2,436 16 View
Hello Every one, I have isolated and sequenced ITS regions for identification of fungi by Sangers method. To construct a phylogenetic tree which is the best tool available in on-line...
09 March 2015 9,547 13 View
Hello Every one, I have done experiments on enzyme production and optimization of production parameters like temp, pH, ...etc. Now I have to optimize the significant parameters by using Response...
07 January 2015 6,299 4 View
What is the best method to isolate/extract fungal genomic DNA? I have tried several methods without the addition of chitinase, but I wasn't successful in this aspect. Please suggest a better...
11 December 2014 7,571 9 View
Hi all,May I know what is the role of acids (HCl, H2SO4, Acetic acid, phosphoric acid, Oxalic acid and nitric acid) in removing lignin/ cellulose/ hemicellulose in lignocellulosic biomass?If we...
13 October 2014 5,932 10 View
Hello Everyone, Please let me know, if there is any single or different methods available for detection of glucose, xylose and xylitol by HPLC equipped with C18 column and UV detector. If not...
20 August 2014 8,832 14 View
How does one quantify total cellulose, hemicellulose, and lignin in biomass before pre-treatment of lignocellulosic biomass?
18 August 2014 2,956 22 View
We are planning to synthesize the new derivatives of natural compound (Ex-curcumin) has not yet synthesized elsewhere. Hence, please let me know the tools to find out all derivatives available for...
18 July 2014 4,943 3 View
We are designing new drugs using online molinspiration program for that we want check the ADMET in silico. What are the standards for ADMET of a designed drug. Are there any software's...
11 July 2014 8,220 27 View
I have isolated some fungi from soil and now I am trying to identify the species. For the identification what are the best primers (Universal/Other) to amplify fungal DNA and where I can get them?...
23 May 2014 6,206 8 View
Is it possible to detect xylitol with Gas Chromatography (GC)? If it's possible what are the conditions for detection of xylitol with GC? We have a GC with capillary column and FID detector. What...
22 May 2014 7,105 5 View
Please let me know how I can detect ethanol in the fermentation media. Is there any specific methodology (injector, column temp, detector temp) for the detection of ethanol in the media? And do we...
27 January 2014 5,228 6 View
I am working on bioethanol production. How can I quantify the reducing sugars percentage (glucose, xylose, arabinose,galactose, etc.) by using HPLC, after pretreatment, and what are the column...
08 July 2013 9,233 3 View
