Gnaneshwar Yadav

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Questions related from Gnaneshwar Yadav

What does this peak in our Bioanalzer profile from Agilent 2100 mean? Why does single peak show up where 28srRNA is expected? Why don't we see 18s?

Hello fellow researchers, Why don't we see two rRNA peaks as is standard? Our sample was a total RNA prep from A549 cell line. We ran our samples on a pico chip for 2100 Agilent bioanalyzer...

29 May 2024 7,641 0 View

What should I consider while doing in situ hybridization for whole zebrafish embryos at 5 days post fertilization using mRNA probe?

Repeated attempts of in situ hybridization have failed for 5 dpf zebrafish embryos. I have treated the embryos with proteinase K at 20 ug/ml concentration for 30 min (I hope this is not too much),...

28 January 2014 3,185 16 View