What is the best strategy to run a 2D-DIGE followed by a "blue silver" stain?

I am performing a 2D-DIGE experiment using 50 ug of protein to each samples. In total, I have 150 ug of protein per gel. I would like to use "blue silver" stain and pick up the spots from gels...

06 December 2013 7,025 6 View