Why isn't IL-2 secreated in Jurkat? What is the way to stimulate T cell?

Hi, guys I have had big problem with Jurkat cell activation. Protocol is as follow: 1. Seed jurkat at 1X 10^5 cell/ml and incubate for O/N 2. Treat jurkat with PHA(sigma, L1668-5MG) at 1, 10,...

30 August 2022 5,485 2 View