Does anybody have a problem of viscosity and clumping solution during Jurkat cell lysis with RIPA buffer?

I'm trying to have a good concentration of a supernatant from Jurkat cells. I've lysed 5 million cells in 500 ul of RIPA BUFFER (almost standard RIPA I would say) and it was already sticky. Than I...

05 May 2012 9,331 4 View