Why did my RIPA buffer precipitate in the Bradford buffer?

Dear all, After protein extraction with a RIPA buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% NP-40, 1% Na-deoxycholate, 0,1% SDS, 1mM EDTA +PIC), I wanted to quantify the yieild by Bradford assay....

19 July 2024 7,821 3 View