Gisele A. Wiezel

3 Questions 4 Answers 0 Followers

Questions related from Gisele A. Wiezel

What happened during Edman sequencing when a N-glycosylated Asn is present in the protein N-terminal?

I'm working with a protein which acording to Uniprot there is an isoform with a N-glycosylated asparagine in the N-terminal region. When I did the amino acid sequencing by Edman degradation, I saw...

01 October 2015 8,522 2 View

Can a threonine residue suffer alkylation?

A threonine residue can be alkylated during an alkylation process using iodoacetamide if overalkylation occurs in the protein?

24 April 2015 2,924 3 View

What is the amount of protein aproximately necessary to determine its amino acid sequence by top-down (MS)? How long does it usually spend?

I'd like to have an idea for a protein with around 58 kDa in terms of mol or mass and assuming there is no mistake during this determination. Thanks in advance

30 August 2014 9,870 8 View