Giovanni De Nola

4 Questions 6 Answers 0 Followers

Questions related from Giovanni De Nola

Are MDCK or HeLa cells sensitive to asparaginase-induced apoptosis?

Dear all I'm trying to study the capacity of a molecule in protecting cells from the pro-apoptotic effect of the asparaginase added in the medium. I have in stock only HeLa cells and MDCK. Does...

04 April 2015 2,554 2 View

How can I determine L-arginine concentration in a sample?

I am using L-arginine in my refolding buffer. After the time required for the refolding to happen I have to dialyse my sample in order to eliminate the arginine that would prevent the binding of...

01 January 2015 7,542 1 View

Experimental technique to determine if protein has free cysteine?

I need to determine if my purified protein has or not a free cysteine, do anyone of you have a good protocol to suggest me? I know that there are a lot of kits made for this purpose but since this...

02 February 2014 6,374 12 View

Does anyone have experience with IB extraction using Lysozyme combined with freeze and thaw bacterial lysis?

I would like to have a good protocol to extract IB using lysozime and cycles of freeze and thaw because I cannot use other mechanical methods as sonication of french press. I would like to know...

01 January 2014 7,810 0 View