Giovanna Revilla

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Questions related from Giovanna Revilla

Is there any issue using 12ug/ml of doxycycline for tet/off system?

Hi, I generated a stable cell line with a shRNA. However, when I tested the optimal dose of DOX to silence the target gene I obtained a working concentration at 12ug/mL... So, as the majority of...

08 March 2023 4,763 1 View

Prestained markers are dissappearing from stain free bioRad gels during the running?

Hi! I am having several problems with the running procedure in western blot. I prepare the acrylamide gels (I use BioRad stain free gels) with fresh APS 10% and TEMED, as I have been always...

21 December 2020 7,172 0 View