How long mouse T cells need to be TCR stimulate using CD3 and CD28 for optimal pS6 staining?

I'm trying to evaluate the contribution of PI3K to S6 phosphorylation using PIK294 to inhibit p110deta after TCR stimulation. I'm wondering if 30 minutes stimulation with cells in incubator would...

01 January 2019 1,413 5 View