Giovane R Sousa

2 Questions 26 Answers 0 Followers

Questions related from Giovane R Sousa

How to correct for wrong products detected in droplet digital PCR (ddPCR) with DNA-specific probes?

I can’t see to find in the literature how a gel electrophoresis or melting curve analysis of ddPCR would be possible. Assuming it is not, I am wondering how can unspecific amplifications then be...

12 December 2020 1,373 1 View

How long mouse T cells need to be TCR stimulate using CD3 and CD28 for optimal pS6 staining?

I'm trying to evaluate the contribution of PI3K to S6 phosphorylation using PIK294 to inhibit p110deta after TCR stimulation. I'm wondering if 30 minutes stimulation with cells in incubator would...

03 January 2019 2,720 4 View