Giorgia Croppi

2 Questions 3 Answers 0 Followers

Questions related from Giorgia Croppi

Does anyone have a protocol to transient transfect HEK293 with 2 plasmids?

I need to transient transfect HEK293 cells with a plasmid containing a luciferase tag and a plamid containing a histamine receptor. I have never done this before so I am looking for a straight...

09 September 2020 947 3 View

Does anyone know if pKD46 system works in E. coli BL21 or DH5alpha to KO a gene?

I need to KO a gene in my bacteria and I'm currently use BL21 strain also for the over expressed one. I've been trying many systems to ko my gene but so far I didn't get any good result and I'm...

04 June 2018 2,252 6 View