Gilda Stefanelli

4 Questions 7 Answers 0 Followers

Questions related from Gilda Stefanelli

Anybody doing EMSA with ethidium bromide?

Hi everybody! I have to do an EMSA to see if my mutations in my protein of interest abolished binding to DNA. Foe lack of time and of permissions of using radioactive labels my boss suggested to...

23 April 2015 4,473 3 View

Why don't my neurons express GFP?

Hi all, I have another mistery to solve. I am trying to infect primary hyppocampal neurons with lentiviruses expressing my protein and then iresGFP. construct work good and are green when...

09 April 2015 1,303 6 View

How much DNA do you usually recover from ChIP?

I am doing ChIP for MeCP2 from total brain Chromatin. I start with 25ug of Chromatin. Assuming my antibody works good, how much DNA do you usually recover from your ChIPs in terms of...

02 April 2015 7,833 9 View

Can anyone help me with SDS-PAGE as the antibody stopped working only in my samples?

I am having serious troubles with my sds pages and hope that somebody can help me. I study a phosphorylated isoform of a protein and my antibody has always detected the protein enough was to put...

28 May 2014 2,020 13 View