Gil Zimran

3 Questions 6 Answers 0 Followers

Questions related from Gil Zimran

What are the odds of dragging viable phage from PCR reaction (was template) and column DNA purification?

we used cDNA library (linear in phage state before plasmid closing) as a template for PCR mediated addition of adaptors.. will the phage survive multiple cycles of boiling? If yes then what can I...

09 January 2017 1,288 1 View

Regarding ADE2 "red/white" selection in yeast two hybrid: Why won't it work on SD minimal medium but only on YPD?

We use the pJ69-4A strain in which endogenous ADE2 is replaced by GAL2uas>ADE2 (y2h interaction dependent expression). Only active ADE2 should yield white colonies while non-active should give...

10 July 2016 2,604 3 View

Is there a protocol for in-medium X-gal dying for y2h screening of cDNA library ?

Can I use the white-blue system to screen a yeast cDNA library without disrupting the cells. Is it possible to apply the Xgal directly in the growth medium? We want to select for events in which...

10 June 2016 4,452 1 View