Why is one plasmid not transfecting HEK 293t cells , while a simliar second plasmid sucessfully transfects the cells with the same protocol?

I ordered and produced a plasmid from Addgene containing PIK3CA and GFP (pHAGE-PIK3CA-C420R (item #116457). I performed mini- and midi preparations and verified plamid production with a...

03 October 2019 4,229 2 View