Giampaolo Milior

5 Questions 6 Answers 0 Followers

Questions related from Giampaolo Milior

Anyone familiar with in vivo staining of microglia?

Hi everyone I am trying to obtain a good staining for live imaging of microglia on brain slice ( human tissue and mouse) In particular I am using a lectin reported to stain blood vessels and...

20 July 2016 1,034 8 View

Can anyone assist me with Tomato lectin- microglia live staining?

Hello! I am looking for some suggestion about the staining on acute rodent brain slices with tomato lectin in order to visualize with 2 photon microscopy the living microglia. I am not sure about...

29 February 2016 4,032 2 View

Anyone familiar with recording of spontaneous spiking activity with patch clamp?

Hi! I'd like to record spontaneous spiking activity from CA1 pyramidal neuron from transverse hippocampal slices of adult mice. Have you ever record it in cell attached configuration? Because i...

09 September 2015 2,616 8 View

Can anyone help me with electrical field potential recordings?

I need help with some aspects the extracellular recordings. I use mouse hippocampal slices (350microm) and mainly my experiments consist of stimulating CA3 shaffer collaterals and record from...

17 June 2014 3,173 8 View

minocycline choice

I want to give my mice minocycline in their drinking water. Does anyone know what kind of minocycline is ok for this purpose? (sigma have the crystalline form for example). Has anyone used...

23 May 2014 778 3 View