Any advice on expressing FOXO1 and RUNX2 cDNA by using pcDNA3.1 topo?

 can I amplify FOXO1 and RUNX2 with proof reading polymerase such as high-quality Taq polymerase. Then, incubate the PCR product with ordinary Taq polymerase to generate A overhang Also,  I would...

10 October 2015 5,085 3 View