Geziel Aguilar

2 Questions 9 Answers 0 Followers

Questions related from Geziel Aguilar

Is it possible to fuse two mCherry sequences (identical DNA sequences) by overlap extension PCR using unique linker regions?

I am trying to generate a 2x mCherry sequence using overlap PCR. The first reaction generates good fragments with overhanging sequences (linkers) but the following 'fusion reaction' does not work....

09 September 2015 5,933 7 View

What is the effect of Aniline blue (in 1M glycine) in Alizarin Red S staining?

I have stained ethanol-fixed plant tissue overnight with aniline blue (1M glycine, pH9) followed by co-staining with Alizarin Red S pH4. Does anyone know the implications of this the co-staining...

01 January 2015 4,092 5 View