Gernot Zissel

2 Questions 7 Answers 0 Followers

Questions related from Gernot Zissel

Why does the molecular size of a GFP-tagged receptor exceed the expected size on a western blot?

Dear all, We tagged a chemokine receptor with GFP and expressed this in an epithelial cell line to analyze receptor trafficking. This works good, the receptor is expressed, we do see receptor...

22 October 2014 8,533 2 View

Problem with the detection of a GPCR by flow cytometry: no signal with PE/Cy5 or Cy7.

I have a problem with the detection of a GPCR by flow cytometry. Using a PE labelled antibody either directly or indirectly using a secondary antibody, I get a nice signal. Because we introduced...

11 October 2012 8,541 2 View