German Jurgens

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Questions related from German Jurgens

How to submit 16S rRNA NGS (Illumina MiSeq) data to the public databases?

Would appreciate any hints/information about submission of NGS data to public databases. In short – manuscript, describing abundance (in percent) of particular microbial groups in different...

19 February 2018 764 5 View

I need to express phytase-like protein in some host. Can I use E.coli BL21(DE3) as expression host?It contains phoA and AppA but was used in one paper

I need to express new unknown phytase-like protein in some host to further study its properties (phytase activity, temp. tolerance etc), but have not much experience in protein expression work. I...

10 May 2017 5,025 7 View

Does anybody have experience with Toyopearl HW-55 resin? What columns could be used with it?

I need to characterize unknown protein by size exclusion chromatography (SEC). What type of column should I buy? I found "C column" from GE Healthcare - somebody have used it for Toyopearl? Any...

01 September 2016 3,814 3 View

Is it possible to extract whole 16S rRNA gene sequence data from WGS Fastq file?

Would appreciate any advice/suggestions on the following task: The sample was Illumina NGS sequenced for 16S amplicon and by WGS approach (I have fastq files for both). From 16S amplicon data, I...

01 January 1970 6,598 1 View