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Questions related from Gergely N. Nagy
I would like to achieve heterologous overexpression of a mycobacterial enzyme in E coli that harbors a Fe-S cluster. Unfortunately, the expressed and purified His-tagged protein apparently lacked...
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Have you experienced/found a publication where positive heat of ligand binding was observed by ITC (ie. endothermic process)? Thanks in advance!
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I tried to clean my Superdex 200 AKTA gel filtration column after an event of protein sample aggregation on the column during gel filtration. Unfortunately, the column seems to be literally...
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