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Dear colleagues! We are facing several problems while trying to establish primary rat endotheliocyte cultures. First of all, cells loose viability after cryoconservation. The freezing medium...
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Hello! I want to isolate RBEC from a rat, I used the protocol - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4208856/. However, I used 5-day old rats instead of adult rats. Subsequently, the...
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