Any tips to increase the yield of BM Mononuclear Cells from the buffy coat that is formed during a Ficoll-gradient centrifugation of a BM sample?

I am trying to isolate Bone Marrow Mesenchymal Stem Cells (BM MSCs)  from BM samples. My first attempt to acquire BM MSCs was based on the retrieval of BM Mononuclear Cells from the BM...

16 December 2014 9,049 5 View