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I am having troubles cutting paraffine embedded mouse bones on a microtome. Fixation and embedding can't completely remove calcium, and I end up with thorn slices all the time. Even additional...
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Hello. I am working with a membrane bound protein, and I've discovered, that after I dissolve and homogenize the tissue in RIPA buffer following by spin down, my protein of interest ends up in the...
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Good day, I am looking for eppendorf tubes that their caps won't fall off during DNA collection with miniprep kit. As for today, each time I will spin the samples with open caps, some of them will...
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I need to take puncture from frozen pig brain slice. What tool and what technique will be most appropriate in order to keep the slice from braking?
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Hello, I am about to perform western on pig brain punctures. I am looking for a good protocol for western, and/or recommendation for appropriate tissue homogenizer. I will be taking 5mm punctures...
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