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Questions related from Gennadiy Fonar
I am having troubles cutting paraffine embedded mouse bones on a microtome. Fixation and embedding can't completely remove calcium, and I end up with thorn slices all the time. Even additional...
24 February 2022 8,893 3 View
Hello. I am working with a membrane bound protein, and I've discovered, that after I dissolve and homogenize the tissue in RIPA buffer following by spin down, my protein of interest ends up in the...
31 January 2022 9,292 2 View
Good day, I am looking for eppendorf tubes that their caps won't fall off during DNA collection with miniprep kit. As for today, each time I will spin the samples with open caps, some of them will...
25 January 2022 918 2 View
I am looking for a b-secretase inhibitor that was used in hiPSC's neuronal culture.
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Good day, I am using human neuronal culture differentiated from hiPSC's. I want to freeze them for future usage. So far I had trouble with keeping the cells alive throughout freezing and...
07 February 2020 6,113 4 View
I need to take puncture from frozen pig brain slice. What tool and what technique will be most appropriate in order to keep the slice from braking?
20 December 2019 3,782 3 View
Hello, I am about to perform western on pig brain punctures. I am looking for a good protocol for western, and/or recommendation for appropriate tissue homogenizer. I will be taking 5mm punctures...
10 December 2019 1,080 0 View
Dear researchers, I am looking for as complete as possible muscarinic AChR inhibitor that passes the BBB. I know that scopolamine is capable of passing the BBB without any problems, but as far...
01 January 1970 6,125 3 View
