Does anyone have experience using pSTAT 1 and 3 antibodies for western blots?

I have been struggling to detect pSTAT 1 and pSTAT3 bands on my western blots. I am running 10% SDS-PAGE gels for approx 90 mins at 100V, transferring at 80V for 90 mins and blocking in BSA. I am...

30 August 2013 6,489 15 View