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Questions related from Geleta Dugassa Barka
I have homologous sequences with more than 80% identity. I wonder these genes might have undergone gene duplication which resulted in their different activity (function), i.e. they are paralogs. I...
19 December 2016 4,007 11 View
I want to search any possible synteny of genes in both coffee and tomato genomes. I have genes sequenced and looking for if these genes maintained the same organization in tomato genome as they...
14 December 2016 671 1 View
Hello everyone. I'm in need of someone's help on how to have exponential phase of amplification curve? I have attached the whole amplification curve and a relatively better amplification curve...
09 December 2015 2,818 3 View
I have run RT-PCR plate to test two primers on two diffrent cDNA (both at conc. of 500ng/microlitre) samples taken at diffrent times. The same concentration of primers (2.5micromolar) resulted...
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The cDNA I have synthesized was from a mixture of mRNA from fungus and plant tissues (as I'm studying interactions). I have a problem in getting the required quantity of cDNA which I can readily...
07 July 2015 1,145 8 View
Is it ok to use DNA molecular weight marker in RNA electrophoresis? Or only RNA molecular weight marker should be used to see RNA integrity?
02 July 2015 7,660 7 View
What is the conc. (and purity of PCR products at 260/280nm) that could be used for sequencing. I have DNA samples amplified by PCR and thinking to use directly for sequencing than using the...
25 October 2014 6,042 15 View
