Geetanjali Sachdeva

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Questions related from Geetanjali Sachdeva

what are the acceptable ct (threshold cycle) values for amplification (qRT-PCR) of a plasma miRNA?

We are optimizing protocols for plasma miRNA isolation and amplification. However for some miRNA, Ct Values fall in the range of 37-40. Negatives are clean. Should we ignore such low Cts as...

07 July 2018 3,741 7 View

Can anyone suggest a simple method to quantify the levels (relative) of a specific protein localized on the apical surface of a cell line?

See above

08 August 2014 3,085 0 View

Can anyone give advice on ShRNA transfected stable clones?

We have been using shRNA plasmids to silence the expression of an endogenous gene. We have realized that some of the stably transfected clones grow in the presence of selection marker containing...

08 August 2014 8,401 7 View

Problems in pelleting of biotinylated cells.

We biotinylate live cells (adherent monolayer of Ishikawa) for 1 hr at 37C. This is followed by quenching and washing with PBS. However after scrapping in PBS, followed by centrifugation at 2000...

01 January 2013 1,405 20 View

How reliable is the chemiluminescent detection of proteins (western blot) for relative quantification?

Our experience tells us that the intensity of immunoreactive bands on luminograms, after chemiluminescent ECL advanced detection does not always correlate with the protein load.

11 November 2012 2,407 7 View