Geetanjali Sachdeva

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Questions related from Geetanjali Sachdeva

Problems in pelleting of biotinylated cells.

We biotinylate live cells (adherent monolayer of Ishikawa) for 1 hr at 37C. This is followed by quenching and washing with PBS. However after scrapping in PBS, followed by centrifugation at 2000...

01 January 2013 1,236 19 View

How reliable is the chemiluminescent detection of proteins (western blot) for relative quantification?

Our experience tells us that the intensity of immunoreactive bands on luminograms, after chemiluminescent ECL advanced detection does not always correlate with the protein load.

16 November 2012 7,670 6 View