Why did human CD4-T cells not get activated by anti-CD3/CD28 beads?

Hi all, I try to activate CD4-T cells isolated from PBMC using Dynabeads® Human T-Activator CD3/CD28 (Invitrogen) with cell/beads ratio 1:1 (1 million cells/well in 6-well plate). After 48 hours...

08 July 2015 6,428 14 View