Gayatri Rajendra Kachh

4 Questions 2 Answers 0 Followers

Questions related from Gayatri Rajendra Kachh

Amplification in Negative Control but no amplification in samples?

Hi, During an experiment I have conducted series of Nested PCR and getting amplification in negative control but not in sample. Every troubleshooting measures have been taken. Also, Positive...

09 September 2018 9,628 3 View

IHHNV 309bp positive control is not working?

Hi, I am working on the screening of Crustacean Viruses. Meanwhile, when I screened my samples for IHHNV (amplicon size 309bp) my positive control is not working. I am getting multiple bands just...

09 September 2018 5,365 2 View

I am getting an intense band in Negative Control PCR for MBV and SWP screening in the Nested step as intense as the Positive Control?

Can anybody please help me out.

12 December 2017 9,909 1 View

How to increase the osmolarity of the cell culture medium

I am working on the cell culture system of A.testudineus. I have to adjust the osmolarity of PBS and medium according to the Serum of the fish. Please suggest me the right procedure.

06 June 2017 4,876 3 View