Gayatri Tandon

4 Questions 2 Answers 0 Followers

Questions related from Gayatri Tandon

Low Ki67 signal in Proliferation Assay?

Hello everyone. I'm performing CD4+Foxp3- (Tcons) and CD4+Foxp3+ (Tregs) cocultures. Where I have titrated the Tregs with same counts of Tcons in several wells to see a suppressive effect of...

27 August 2021 6,971 2 View

Low CD4+ signal in functional suppression assay. Could cell death be a reason or weak staining??

Currently I'm performing functional suppression assays with coculturing Tregs (CD4+YFP+) and Tcon (CD4+)cells for 72 hrs. I see very weak signal of CD4+ post harvesting. What could be the reason?...

05 August 2021 6,293 0 View

Can I directly gate the live cell population to check the dye dilution in my functional suppression assay?

I have Treg (CD4+YFP+) cells and Tcon (CD4+) cells in coculture. The proliferation marker is Cell Trace Violet. Post harvesting, I stain my samples for live dead staining with blue fluorescent...

05 August 2021 1,470 0 View

Stimulation of spleenocytes with MOG peptide in EAE model

In my EAE model, I want to stimulate mouse spleenocytes with MOG peptide in vitro to study cytokine release by flowcytometry. I plan to do it in the presence of Brefeldin and Monensin. I checked...

01 January 1970 4,487 0 View