Gayathri Subramanian

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Questions related from Gayathri Subramanian

Does passage number affect the localization of probes during RNA FISH?

Does increase in passage number cause your probes to localize in nucleolus in the case of cancer cell line?

09 July 2020 5,130 0 View

How do you exactly determine the annealing temeperature while splicing two gene sequences in the first annealing cycle of overlap extension pcr?

In OE PCR, I have to stitch two gene sequences, so the annealing temperature in the first cycle would depend on the overlap reagion between two products designed with appropriate primers or would...

15 March 2020 3,001 3 View