Why Lentiviral Plko.1 shRNA plasmid trasduction not working despite in high PURO pressure?

Hi! I am trying to knockdown AR protein in a prostate cancer cell-line and I have used a commercial Lentiviral shRNA particles (pLKO.1 plasmid Titre 10^6). Briefly, I seeded 20,000 cells per well...

12 December 2018 648 4 View