Gaurav Joshi

3 Questions 4 Answers 0 Followers

Questions related from Gaurav Joshi

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

24 February 2021 5,931 1 View

Will some bases between GFP-P2A-few bases-GOI work?

We are cloning a gene of interest (GOI) after P2A that is preceded by GFP. After P2A sequence there is a gap of few base pairs followed by gene of interest but this is in-frame with P2A and is...

17 February 2019 6,059 5 View

Time limit for skin biopsy digestion using collagenase for fibroblast extraction?

I digested skin biopsy using collagenase for fibroblast culture. Can keeping it for more than 12 hours overdigest it?

15 May 2017 7,622 3 View