Gary Steven McDowell

3 Questions 9 Answers 0 Followers

Questions related from Gary Steven McDowell

Does anyone have images of HGEM Xenopus tadpole eyes?

I'm trying to score transgenic HGEM (heat-shock green eyed monster, with γ-crystallin promoter driving expression of GFP) Xenopus tadpoles, but as tadpole eyes are already quite bright, it's...

12 September 2014 876 1 View

How targeted can you make one-cell Xenopus embryo injections?

Xenopus eggs are large and if I'm injecting 10 nl of mRNA solution into a particular site, how targeted will that injection be? Is there any idea how rapidly mRNA might diffuse throughout the...

05 July 2014 6,359 4 View

What's the best way of measuring TMT/iTRAQ labelling efficiency?

I can't seem to find any universally-accepted method for measuring TMT or iTRAQ labelling efficiency. Some check just for any dynamic modifications at a peptide level; some compare fully labelled,...

21 March 2013 3,727 2 View