I have over 1000 aligned gene sequences and am trying to design 1-5 qPCR primers to target them. How should I go about doing this?

I have a large set of cyanobacterial gene sequences aligned and am trying to find potential primer sequences for them. I would like to create a multiplex qPCR with no more than 5 primer sets. The...

28 February 2018 9,422 3 View