Garima Singh

9 Questions 8 Answers 0 Followers

Questions related from Garima Singh

How to do median and quantile normalization of microarray data in GEO which does not have .CEL files?

I want to normalize the GSE data which is in .txt format i.e non-normalized. How to do median and quantile normalization of this .txt file using R? Your help is appreciated Thanks

02 February 2017 10,039 0 View

How to calculate number of Calcium ions during MD simulation?

I want to add 1mM calcium ions inside my protein simulation box after knowing the number of water molecules in water box. How to calculate the calcium ions? What are the steps in GROMACS to follow...

08 August 2016 9,026 4 View

MD Simulation in GROMACS 5.0 at a specific pH?

I'm working on a protein which is active at pH =5. I want to simulate this protein with a mutation at pH 5. How to give this parameter?

07 July 2016 8,378 4 View

Next Generation Sequencing Training?

There is a highly recognized company in the field of Life Sciences/ Bioinformatics- Shodhaka, which is planning to organize high throughput training in Next Generation Sequencing, but they need a...

07 July 2016 3,102 0 View

Is 20ns simulation enough to study 317 amino acid length protein in GROMACS?

My protein is a cysteine protease of 317 aa with a single point mutation. I want to simulate the mutated structure. What should be the time length ?

07 July 2016 3,462 8 View

Can anyone suggest a pancreatic cell line expressing CASR and SPINK1 gene?

I need normal Pancreatic cell lines

09 September 2015 1,470 0 View

Is there a database of pre-validated Real-Time PCR primers?

I want a database where experimentally validated Q-PCR PRIMERS are available . I am using SYBR Green for real-time PCR. Waiting for your valuable answers.

03 March 2015 4,686 2 View

Is it right to design primers for Real-Time PCR (SYBR-Green) by using coding the sequence as the template?

I downloaded the Coding Sequence s from NCBI and designed primers using Primer-BLAST (NCBI)

03 March 2015 5,781 5 View

Which is the most validated gene to be used as a control in Q-PCR ?

My Experiment sample is pancreatic tissue. Please help me in deciding the control gene to be analysed by Q-PCR.

03 March 2015 7,609 19 View