Garima Joshi

2 Questions 2 Answers 0 Followers

Questions related from Garima Joshi

Why do bands stay on sds page gel even after performing western blot?

I have performed transfection of n and p genes of ppr virus in vero cells.. Later i went for sds page followed ny westwrn blotring... After staining my page gel i could still see some bands on my...

25 January 2018 4,283 9 View

Why can't mammalian proteins be observed in sds page?

I'm trying to transfect N and P proteins of PPR virus in mammalian expression system. Why can't the proteins be viewed on sds page, why is western blotting necessary to observe the expressed protein?

24 January 2018 8,213 2 View