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I differentiated THP-1 cells to macrophages using PMA(100ng/ml) and then used lentivirus to deliver shRNA to knockdown my target genes. Unfortunately, it was too difficult to be transfected...
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I would like to discover the effect of my target gene on macrophage polarizing. I have used lentivirus to infect PMA-treated THP-1 to konckdown my gene, but it was too difficult to be infected....
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