Gangotri Patra

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Questions related from Gangotri Patra

Hi,I have been trying to transfect MDA MB 231 cell lines since a while, I tried with lipofectamine LTX as well as lipofectamine 3000.Please suggest?

Hi,I have been trying to transfect MDA MB 231 cell lines since a while, I tried with lipofectamine LTX as well as lipofectamine 3000.Please suggest how to proceed with this cell line and what is...

03 June 2024 1,609 0 View

The size of my protein is 5KD, I have tried to detect it using 20% SDS gel but didnt get it. Please suggest me how to proceed with my work?

I have 2 proteins-one is 19KD and other is 5KD. I tried detecting both of them through western blot using 20% SDS gel. I transferred them into 0.45 PDVF membrane for 100mins, I could detect the...

20 May 2024 3,088 0 View

The size of my protein is 5KD, I have tried to detect it using 20% SDS gel but didnt get a band. Please suggest me how to proceed with my work?

I have 2 proteins-one is 19KD and other is 5KD. I tried detecting both of them through western blot using 20% SDS gel. I transferred them into 0.45 PDVF membrane for 100mins, I could detect the...

01 January 1970 5,728 3 View

Hi,I have been trying to transfect MDA MB 231 cell lines since a while, I tried with lipofectamine LTX as well as lipofectamine 3000.Please suggest?

Hi,I have been trying to transfect MDA MB 231 cell lines since a while, I tried with lipofectamine LTX as well as lipofectamine 3000.Please suggest what are the best methods to transfect this cell...

01 January 1970 1,827 3 View