Ganga Viswanath

5 Questions 31 Answers 0 Followers

Questions related from Ganga Viswanath

How might one store fungal cultures for longer period?

I have maintained the fungal cultures in agar slants. But certain fungal cultures get contaminated due to water droplets when stored at 4 degree Celsius and provided cultures need to be...

27 August 2015 5,175 25 View

How can I completely remove DNA in my RNA sample?

I am isolating a total RNA from bacterial sample for real time PCR studies. But i am facing a problem with the DNA contamination. I treated the final RNA sample with DNases enzyme. I had done a...

13 September 2014 6,168 14 View

Does anyone know a selective medium for specific bacterial groups?

I am working in the isolation of specific groups of bacteria such as Stenotrophomonas, Cobetia, Sphingomonas, Artthrobacter from sediment and rhizosphere source. I have to be more specific in...

03 July 2014 9,630 0 View

How to overcome the problem in real-time PCR melting curve variation between the same replicates?

I have started with real time experiments. When I do a melting curve analysis for the primers, I get a different Tm for the same sample in the replicates. But there is no primer dimer, only single...

09 April 2014 9,931 15 View

Does anyone have a protocol for purification of acyl homoserine lactones (AHLs)?

I am working on the AHL producing rhizobacteria. Initial detection of AHL molecules by reverse phase TLC was carried out. I need to know which type of molecules the spots detected by the reporter...

07 March 2014 540 3 View