Gang Yu

2 Questions 2 Answers 0 Followers

Questions related from Gang Yu

How can I improve the protein solubility in insect cells?

I am trying to express a LRR (leucine rich repeat) protein in insect cells, but the proteins appeared to be insoluble. Are there any methods to improve the protein solubility in insect...

08 August 2015 2,385 4 View

Is there a way to disrupt the protein aggregate?

I used HisTrap to purify a His-MBP tagged protein, and then dialyzed the protein against 20mM Tris-HCl, 150mM NaCl, pH7.4. The protein is pretty soluble and I concentrated to around 10mg/ml, then...

10 October 2014 9,437 9 View