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Questions related from Ganesh Pai B
Abcam-#ab6326 (Rat), BD Pharmingen-#347580 (Ms) is what we use currently. Due to supply disruptions, we are forced to change the antibodies and find alternatives for the same. Any help in this...
17 August 2024 1,947 0 View
I would want to perform a Mass spectrometric analysis of co-immunoprecipitated proteins after having pulled down a specific protein using magnetic beads (dyna beads) or agarose conjugated with...
01 December 2021 1,960 0 View
Do methanol fixation and paraformaldehyde alter the ability to detect the foci of 53BP1 in cells? Also, I would be interested to know if the protocol of pre-extraction (using the solution -25 mM...
17 July 2021 599 0 View
In the absence of filter individually ventilated cages, is there a possibility that nude mice be housed in filter-top cage in rooms meant for animal rearing without any sophisticated arrangements/...
02 February 2021 7,633 3 View
I observe that a certain drug treatment induces autophagy in cancer cells. I am trying to distinguish the role of autophagy, to find out whether it aids in cell survival or autophagy activation...
22 August 2020 5,357 5 View
PBS had turned cloudy upon autoclaving. I used milliQ water for preparation of PBS with proper ingredients but it became cloudy upon autoclaving.
27 September 2016 7,221 15 View
How long can a freeze vial of MCF7 cells or any other cell line be stored at -70 degree celsius before transferring to liquid nitrogen? Freeze vial for long term storage
16 September 2016 3,513 8 View
Bacterial pellet with very little medium was stored at 4 degrees.
31 August 2016 4,340 6 View
