Ganesh Pai B

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Questions related from Ganesh Pai B

Can anyone suggest alternative to the DNA fiber antibodies listed below?

Abcam-#ab6326 (Rat), BD Pharmingen-#347580 (Ms) is what we use currently. Due to supply disruptions, we are forced to change the antibodies and find alternatives for the same. Any help in this...

17 August 2024 1,937 0 View

Can anyone suggest a working protocol for performing Mass spectrometric analysis following Co-Immunoprecipitation (Co-IP) of a protein using Magbeads?

I would want to perform a Mass spectrometric analysis of co-immunoprecipitated proteins after having pulled down a specific protein using magnetic beads (dyna beads) or agarose conjugated with...

01 December 2021 1,949 0 View

Would there be a difference in the detection of 53BP1 foci upon usage of different fixation methods or the usage of pre-extraction buffer?

Do methanol fixation and paraformaldehyde alter the ability to detect the foci of 53BP1 in cells? Also, I would be interested to know if the protocol of pre-extraction (using the solution -25 mM...

17 July 2021 590 0 View

Can I house immunodeficient nude mice in filter-top cages without any ventilation system apart from the filter itself?

In the absence of filter individually ventilated cages, is there a possibility that nude mice be housed in filter-top cage in rooms meant for animal rearing without any sophisticated arrangements/...

02 February 2021 7,617 3 View

How do I differentiate between autophagic cell death and autophagy activation leading to survival?

I observe that a certain drug treatment induces autophagy in cancer cells. I am trying to distinguish the role of autophagy, to find out whether it aids in cell survival or autophagy activation...

22 August 2020 5,340 5 View

Why does PBS turn cloudy upon autoclaving, sometimes?

PBS had turned cloudy upon autoclaving. I used milliQ water for preparation of PBS with proper ingredients but it became cloudy upon autoclaving.

27 September 2016 7,209 15 View

How long can a freeze vial of MCF7 cells or any other cell line be stored at -70 degree celsius before transferring to liquid nitrogen?

How long can a freeze vial of MCF7 cells or any other cell line be stored at -70 degree celsius before transferring to liquid nitrogen? Freeze vial for long term storage

16 September 2016 3,495 8 View

For how many days can a bacterial culture pellet be stored at 4 degree celsius in the pellet form with very little medium?

Bacterial pellet with very little medium was stored at 4 degrees.

31 August 2016 4,334 6 View