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Questions related from Ganesh Pai B
I would want to perform a Mass spectrometric analysis of co-immunoprecipitated proteins after having pulled down a specific protein using magnetic beads (dyna beads) or agarose conjugated with...
01 December 2021 1,872 0 View
Do methanol fixation and paraformaldehyde alter the ability to detect the foci of 53BP1 in cells? Also, I would be interested to know if the protocol of pre-extraction (using the solution -25 mM...
17 July 2021 488 0 View
In the absence of filter individually ventilated cages, is there a possibility that nude mice be housed in filter-top cage in rooms meant for animal rearing without any sophisticated arrangements/...
02 February 2021 7,554 3 View
I observe that a certain drug treatment induces autophagy in cancer cells. I am trying to distinguish the role of autophagy, to find out whether it aids in cell survival or autophagy activation...
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PBS had turned cloudy upon autoclaving. I used milliQ water for preparation of PBS with proper ingredients but it became cloudy upon autoclaving.
27 September 2016 7,172 15 View
How long can a freeze vial of MCF7 cells or any other cell line be stored at -70 degree celsius before transferring to liquid nitrogen? Freeze vial for long term storage
16 September 2016 3,349 8 View
Bacterial pellet with very little medium was stored at 4 degrees.
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