(Sanger Sequencing) What is causing a 'T' artifact in every chromatogram of my CRISPR iPSC colonies?

I followed a regular CRISPR-cas9 transfection protocol, manually picked colonies into a 96-well plate and performed nested PCR which I then sent in for sequencing. In my previous 3 runs, I have...

12 July 2019 2,899 4 View