Gaia Pigna

4 Questions 11 Answers 0 Followers

Questions related from Gaia Pigna

Do you need to extract chloroplasts to stain cpDNA with DAPI?

I'm looking for a protocol that avoid chloroplasts extraction to perform the DAPI staining of the cpDNA. Since I've found only protocols that require chloroplast extraction I'm wondering if it is...

21 January 2015 6,993 1 View

Hard callus formation: how to avoid it?

I’m working on callus induction of different genotypes of Sugar beet; I’ve a problem one of this genotype produced hard callus. I don’t know how to avoid this phenomenon, can you help me?

15 April 2013 9,853 7 View

Does anyone try to induce callus formation from root explants of herbaceous plants like phragmites, miscanthus, zingiber?

I’m working on callus induction of Arundo donax (giant reed) starting from different explants. Have you ever tried to induce callus formation starting from roots of herbaceous plants? Thanks’ in...

14 March 2013 9,272 1 View

Does anybody know how to reduce browning of sugarbeets in vitro colture?

I'm working on callogenesis of three different genotypes of sugar beet. I started from hypocotyls and leaf explants of three genotypes on a MS, they turned from green to brown in 3 weeks. I've...

13 March 2013 665 18 View