Gabriella Di Felice

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Questions related from Gabriella Di Felice

Can anyone kindly provide me with protocols and practical suggestions for rat peritoneal macrophages?

I would set up the procedure of peritoneal washing to obtain rat resident macrophages (without previous recruitment and activation). We have had also difficulties in recovering adherent cells...

04 April 2014 7,183 11 View

Freezing BMDC at the end of the differentiation culture in GM-CSF (day 8-10): do you have experience about any effect on BMDC maturation and function?

We prepare mouse bone marrow-derived DC according to the protocol of Lutz (J Imm Meth 223,1999:77–92). I am in particular interested in CD86 expression and inflammatory cytokine production

03 March 2013 2,981 1 View