Gabriele Colozza

4 Questions 10 Answers 0 Followers

Questions related from Gabriele Colozza

Does anyone have experience with Xenopus Notum protein?

I cloned a Xenopus Notum homolog. Its sequence is identical to the published xNotum. It is tagged with a 3X Flag. When I co-inject it together with Wnt8 into ventral blastomeres of Xenopus...

08 August 2017 6,521 2 View

Does anyone have hLRP6 in pDonr221?

I am looking for human LRP6 in a pDonr221 vector (or any other vector compatible with Gateway LR Recombination). Does anybody have it and is ready to share a little bit of it? Do you also have a...

02 February 2015 4,724 3 View

How can I find different alleles in Xenopus laevis?

I am trying to design a morpholino to knockdown a Xenopus laevis gene. Since laevis is allotetraploid, I want to find all the possible alleles of this gene and possibly design one morpholino (or...

01 January 2015 1,130 6 View

Does anyone have a protocol for gst pulldown in Xenopus embryos?

I would like to express a GST-tagged protein in Xenopus embryos. After lysis of the embryos, I would like to couple it to a glutathione matrix, and use it as a bait to assay interactions with...

01 January 2015 7,150 0 View